A3051 is a potent and orally active inhibtor of CXXC5-DVL extracted from patent WO2020079569, has an IC 50 of 63.06 nM. A3334 can be used for the research of high fat diet (HFD)-induced and methionine-choline deficient diet (MCD)-induced phenotypes such as obesity, diabetes, and NASH.

A3334 (1-10 μM) efficiently inhibits the adipogenic differentiation of 3T3-L1 preadipocytes via the Wnt/β-eatenin signaling pathway[1]. A3334 (1-10 μM; 24 h) enhances the TOPFlash reporter activity in HEK293-TOP cells[1].

A3334 (25 mg/kg; p.o. once daily for 16 weeks) has anti-obesity effects in the HFD mice, and has no effect on mice fed with normal diet[1]. A3334 (25 mg/kg; p.o. once daily for 5 days) significantly reduces fasting glucose and the levels of glucose tolerance (GTT) and insulin tolerance (ITT) in serum in mice[1]. A3334 (25 mg/kg; p.o. once daily for 3 weeks) significantly abolishes hepatosteatosis and the increased levels of alanine transaminase (ALT) and aspartatetransaminase (AST) in mice[1]. Animal Model: Male C57BL/6N mice (6-week-old) are fed on the high fat die (HFD) for 16 weeks[1]Dosage: 25 mg/kg Administration: P.o. daily for 16 weeks Result: Not observed the HFD-induced body weight gain and abdominal obesity. Reduced the level of triglyceride and total cholesterol and increased the level of HDL-cholesterol. Suppressed the increase in adipocyte cell sizes and enhancement of inflammation.

854171-31-6

C17H13N3O3

307.3

A3334

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years