KKL-35 是一种具有广谱抗生素活性的反式翻译抑制剂，IC50值为 0.9 μM。

KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity.

KKL-35 inhibited tagging of DHFR-ns. Dose-response experiments showed that KKL-35 inhibited tagging with an IC50 = 0.9 μM. KKL-35 prevented the growth of WT S. flexneri with a minimum inhibitory concentration (MIC) of 6 μM, and the addition of KKL-35 to a growing culture of S. flexneri stops growth [1]. Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 remained equally active against L. pneumophila mutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophila in human macrophages at several stages of infection [2].

Overnight cultures of S. flexneri 2a 2457T, B. anthracis, M. smegmatis, JW5503, and NSR253 were grown in lysogeny broth (LB). Cultures were diluted to a final inoculum of ～5 × 10^5 cfu/mL in 96-well microtiter plates, compounds were added at appropriate concentrations, and growth was observed after 24-h incubation at 37 °C. The MIC was determined by the concentration of the compound in the last well that showed no bacterial growth. For MBC assays, 10 μL from wells containing the MIC, 2× MIC, and 4× MIC of each compound was diluted 10-fold, spotted on LB plates, and grown overnight at 37 °C. An inhibitor was scored as bactericidal if it resulted in 1,000-fold reduction in colony-forming units per milliliter from the original inoculum [1].

865285-29-6

C15H9ClFN3O2

317.7

KKL-35

DMSO:5 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years