O-Propargyl-Puromycin is a potent inhibitor of protein synthesis.

O-propargyl-puromycin (OP-puro).?OP-puro forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition.?Unlike methionine analogs, OP-puro does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.?This strategy should have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo

In the small intestine, translation is strongest in cells in the crypts and at the base of intestinal villi, consistent with the high proliferative and secretory activity of these cells.?The stain is particularly strong in Paneth cells, which are located close to the base of the crypts and are filled with secretory vesicles.?The intense O-propargyl-puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated, O-propargyl-puromycin-conjugated secretory proteins are translocated into the endoplasmic reticulum (ER) lumen[1].

1416561-90-4

C24H29N7O5

495.53

O-Propargylpuromycin;O-Propargyl-Puromycin

DMSO:31 mg/mL (62.56 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years