Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.

The first legumain fluorogenic substrate Cbz-Ala-Ala-Asn-AMC based on the P3-P2-P1 sequence Ala-Ala-Asn. The synthetic substrate Cbz-Ala-Ala-Asn-AMC is effectively cleaved by S. mansoni legumain and human legumain with K m s of 90 and 80 uM respectively[2][3].

149697-16-5

C28H31N5O8

565.583

Cbz-Ala-Ala-Asn-AMC;Z-Ala-Ala-Asn-AMC;Z-Ala-Ala-Asn-AMC

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years