Aglafoline inhibits in a concentration-dependent manner the aggregation and ATP release reaction induced in washed rabbit platelets by PAF (platelet-activating factor). The IC50 values of Aglafoline on PAF (3.6 nM)-induced platelet aggregation were about 50 μM.

Aglafoline also inhibits [3H]PAF (3.6 nM) binding to washed rabbit platelets (IC50: 17.8 μM). The concentration-response curve of PAF-induced platelet aggregation was shifted to the right by Aglafoline (pA2: 5.97; pA10: 5.04). Although thromboxane B2 formation caused by collagen and thrombin was partially suppressed by Aglafoline, thromboxane B2 formation caused by ionophore A23187 and arachidonic acid was not affected. Aglafoline inhibited the [3H]inositol monophosphate formation caused by PAF but not that caused by collagen or thrombin in the presence of indomethacin (20 μM).

The cAMP content of washed rabbit platelets was not affected by Aglafoline. Rat femoral intravenous administration of Aglafoline (10 mg/kg) did not affect blood pressure. However, Aglafoline (10 mg/kg) both prophylactically and therapeutically antagonized PAF (2.5 μg/kg)-induced hypotensive shock in rats. Intravenous PAF (30 ng/kg) caused severe bronchoconstriction in guinea pigs. This effect was completely blocked by Aglafoline.

143901-35-3

C28H28O8

492.52

(-)-Methyl rocaglate;Rocaglamide U;Aglafolin;Aglafoline

DMSO:21.43 mg/mL (43.51 mM),Need ultrasonic
Ethanol:100 mg/mL (203.04 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years