L-ascorbic acid 2-phosphate can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation. L-ascorbic acid 2-phosphate is a long-acting vitamin C derivative that can stimulate collagen formation and expression.

A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect .?L-Ascorbic acid 2-phosphate (50 μM-250 μM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity.?The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex.L-Ascorbic acid 2-phosphate ?significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) ?achieves only weak growth stimulation.

23313-12-4

C6H9O9P

256.1

2-Phospho-L-ascorbic acid;维生素C磷酸酯;L-Ascorbic acid 2-phosphate

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years