Lin28-let-7a antagonist 1 对 Lin28A-let-7a-1 相互作用的 IC50 为 4.03 μM，对 Lin28-let-7a 相互作用有明显的拮抗作用。

Lin28-let-7a antagonist 1, with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction，and shows a clear antagonistic effect against the Lin28-let-7a interaction.

When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28.Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28A-let-7a-1 interaction with an IC50 of 4.03 μM.?Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency.?Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA.?

2024548-03-4

C31H29N5O7

583.59

Lin28-let-7a antagonist 1

DMSO:60 mg/ml (102.81 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years