CelGreen 是一种具有独特参与性的荧光染料，可与所有dsDNA双螺旋沟区域结合。在游离状态下，侧链和CelGreen发出微弱的荧光，但一旦与双链DNA结合，荧光就会大大增强。因此，CelGreen的荧光信号强度与双链DNA的量有关，可以根据荧光信号检测PCR系统中双链DNA的量。

CelGreen is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

GelGreen for Electrophoresis In Gel Staining (Pre-staining, same method as EB) Prepare a agarose solution and heat till it dissolves. GelGreen Nucleic Acid Staining solution is diluted from the GelGreen Nucleic Acid Stain 1:10,000 prior to pouring the gel. Since good thermal stability, GelGreen can be added directly to hot agarose solution. Shake to ensure being fully mixed flowing by cast the gel, Run gel and view results by UV projector.

TD0003

CelGreen nucleic acid gel stain *10,000× concentrate in water*

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years