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EF-5
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
EF-5图片
CAS NO:152721-37-4
包装与价格:
包装价格(元)
1 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
EF5
2-Nitroimidazole
产品介绍
EF-5 是一种能够用于鉴定细胞缺氧的缺氧标记试剂。

产品描述

EF-5 is a agent of hypoxia labeling. It is used to identify hypoxia in cells.

体外活性

EF-5 binding and metabolic reduction of the prodrugs are highly correlated in a panel of 14 hypoxic tumor cell lines. Overexpression of CYPOR causes similar 2- to 4-fold increases in EF-5 binding and metabolic reduction of tirapazamine and CEN-209 in SiHa and HCT116 cell lines. Which is similar enhancement of γH2AX formation.[1].

体内活性

EF-5 binding is a promising stratification biomarker for benzotriazine-N-oxide bioreductive prodrugs. Binding and detection using a monoclonal antibody in 9L gliomas is specific and oxygen dependent, following intravenous injection of EF-5. Modification of tumor hypoxia causes similar changes to the bioreductive activation of both agents, resulting in a strong correlation between EF-5 binding and CEN209-induced DNA damage at the individual tumor level[1]. Detection of binding using fluorescence microscopy can be performed on frozen tissues; tissue sections can be counterstained with haematoxylin and eosin for light microscopic analysis. The distribution of hypoxia in a tumor can be inferred by examining individual tumor cells using flow cytometric techniques[2].

Cas No.

152721-37-4

分子式

C8H7F5N4O3

分子量

302.16

别名

EF5;2-Nitroimidazole

储存和溶解度

DMSO:125 mg/mL (413.69 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years