INH1 通过直接结合Hec1，特异性干扰 Hec1/Nek2 的相互作用。它在体内外均表现出良好的抗肿瘤活性。

INH1 is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction.

腹腔注射100 mg/kgINH1抑制含有MDA-MB-468人乳腺癌异种移植物的小鼠中乳腺肿瘤生长.

INH1有效抑制人乳腺癌细胞增殖,GI50为10-21 μM。此外,INH1也会通过损害纺锤体监测点调控的Hec1/Nek2通路,引发细胞杀伤活性。

Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments).

Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference)

313553-47-8

C18H16N2OS

308.4

IBT13131

DMSO:30.8 mg/mL (100 mM)
Ethanol:3.1 mg/mL (10 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years