Suberosin 可分离自Plumbago zeylanica 中，通过调节NF-AT和NF-Κb，抑制PHA-诱导的 PBMC细胞增殖并捕获细胞周期G1过渡到 S期，具有抗炎和抗血凝的活性。

Suberosin exhibits anti-inflammatory, and anticoagulant activities, it also shows biting deterrent activity against Aedes aegypti, it may be useful for use as mosquito larvicidal agent.

Suberosin suppressed PHA-induced PBMC proliferation and arrested cell cycle progression from the G1 transition to the S phase. Suberosin suppressed, in activated PBMC, transcripts of interleukin-2 (IL-2), interferon-gamma (IFN-gamma), and cyclins D3, E, A, and B. DNA binding activity and nuclear translocation of NF-AT and NF-kappaB induced by PHA were blocked by suberosin. Suberosin decreased the rise in intracellular Ca2+ concentration ([Ca2+]i) in PBMC stimulated with PHA. Suberosin did not affect phosphorylation of p38 and JNK but did reduce activation of ERK in PHA-treated PBMC. Pharmacological inhibitors of NF-kappaB, NF-AT, and ERK decreased expression of mRNA for the cyclins, IL-2, and IFN-gamma and cell proliferation in PBMC activated by PHA.

Proliferation of PBMC in culture was measured by uptake of 3H-thymidine; production of cytokines and cyclins by Western blotting and RT-PCR. Transcription factors NF-AT and NF-kappaB were assayed by immunocytochemistry and EMSA

581-31-7

C15H16O3

244.29

软木花椒素

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years