Autophagy inducer 4 是一种基于 Magnolol 的曼尼希碱衍生物，可用作抗癌剂。Autophagy inducer 4 通过诱导Autophagy" style="display: inline; color: #c13a36">自噬 (Autophagy) 来抑制癌细胞。Autophagy inducer 4 对 T47D 细胞的毒性是 Magnolol 的 76 倍。Autophagy inducer 4 对 T47D 和 Hela 癌细胞的迁移也有抑制作用。

Autophagy inducer 4 is a Magnolol-based Mannich base derivatives, which can be used as an anticancer agent. Autophagy inducer 4 suppresses cancer cells via inducing autophagy. Autophagy inducer 4 has 76-fold improvement in cytotoxicity against T47D cells compared with Magnolol. Autophagy inducer 4 also possesses suppressive effects on migration of T47D and Hela cancer cells [1].

Autophagy inducer 4 (compound 3p) (0-10 μM; 72 hours) displays highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines [1]. Autophagy inducer 4 (40-80 μM; 0-36 hours) significantly increases GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells [1]. Autophagy inducer 4 (0-80 μM; 0-36 hours) increases the transformation of LC3-I into LC3-II in a dose- and time-dependent manner [1]. Cell Proliferation Assay Cell Line: T47D, MCF-7 and Hela cells [1] Concentration: 0-10 μM Incubation Time: 72 hours Result: Displayed highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines with IC 50 values of 0.91, 3.32 and 1.71 μM, respectively. Cell Autophagy Assay Cell Line: GFP-LC3-HEK293 [1] Concentration: 40, 60 and 80 μM Incubation Time: 0, 12, 24 and 36 hours Result: Significantly increased GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells. Western Blot Analysis Cell Line: Hela, T47D and HEK293 [1] Concentration: 0, 5, 10 and 20 μM in T47D and Hela; 0, 40, 60 and 80 μM in HEK293 Incubation Time: 0, 12, 24 and 36 hours Result: Increased the transformation of LC3-I into LC3-II in a dose-dependent manner, and enhanced the expression of LC3-II in a time-dependent manner.

2486455-03-0

C32H37NO6

531.64

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years