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Kalii Dehydrographolidi Succinas
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
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CAS NO:76958-99-1
包装与价格:
包装价格(元)
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
穿琥宁
Ddhads
Dehydroandrographolide Succinate Potasium Salt
产品介绍
Dehydroandrographolide Succinate Potasium Salt 从中药穿心莲中提取得到,具有免疫刺激、抗感染和抗炎作用,对病毒性肺炎和病毒性上呼吸道感染具有潜在的研究价值。

产品描述

Dehydroandrographolide Succinate Potasium Salt is a natural compound with anti-virus infection activity.

激酶实验

In vitro kinase assay is based on a filter binding assay, using the recombinant GST-fused kinase domains expressed in baculovirus and purified over glutathione-sepharose, γ-[33P]ATP as the phosphate donor, and poly(Glu:Tyr 4:1) peptide as the acceptor. Each GST-fused kinase is incubated under optimized buffer conditions [20 mM Tris-HCl buffer (pH 7.5), 1-3 mM MnCl2, 3-10 mM MgCl2, 3-8 μg/mL poly(Glu:Tyr 4:1), 0.25 mg/mL polyethylene glycol 20000, 8 μM ATP, 10 μM sodium vanadate, 1 mM DTT] and 0.2 μCi γ-33P ATP in a total volume of 30 μL in the presence or absence of a test substance for 10 min at ambient temperature. The reaction is stopped by adding 10 mL of 250 mM EDTA. Using a 384-well filter system, half the volume is transferred onto an Immobilon-polyvinylidene difluoride membrane. The membrane is then washed extensively and dried, and scintillation counting is performed. IC50s for compounds are calculated by linear regression analysis of the percentage inhibition[1].

Cas No.

76958-99-1

分子式

C28H35KO10

分子量

570.676

别名

穿琥宁;Ddhads;Dehydroandrographolide Succinate Potasium Salt

储存和溶解度

DMSO:10 mg/mL(17.5 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years