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Ac-DEVD-AFC
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ac-DEVD-AFC图片
CAS NO:201608-14-2
包装与价格:
包装价格(元)
1 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
500 mg电议
1 mL*10 mM(in DMSO)电议

产品介绍
Ac-DEVD-AFC 是荧光底物 (λex=400 nm, λem=530 nm)。

产品描述

Ac-DEVD-AFC is a fluorogenic substrate

体外活性

Caspase activation was measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC). We show that this substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-amino-4-trifluoromethyl coumarin (AFC). Following cell disruption, released AFC was separated on HPLC and detected by fluorescence. The appearance of AFC in cells was blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases were responsible for the cleavage. Caspase activity was first noted after about 2 h of incubation with doxorubicin or dactinomycin, the production of AFC being linear with time afterward[1].

Cas No.

201608-14-2

分子式

C30H34F3N5O13

分子量

729.619

储存和溶解度

DMSO:40 mg/mL (54.82 mM)
H2O:insoluble
Powder: -20°C for 3 years
In solvent: -80°C for 2 years