Leupeptin hemisulfate 是可透过膜的巯基蛋白酶抑制剂，能够抑制Cathepsin B、Cathepsin H、Cathepsin L的活性，及阻断两性溶酶体的融合。它也具有抗炎活性。

Leupeptin hemisulfate is a reversible, competitive serine/cysteine protease inhibitor. It is an orally active, antioxidant and anti-inflammatory agent.

Leupeptin hemisulfate能够恢复细胞悬浮培养液中高达50%的乙型肝炎表面抗原的表达。由各种放线菌产生的亮肽素强烈抑制蛋白水解。Leupeptin hemisulfate保护微管蛋白在分离过程中免受内源性蛋白质水解酶的破坏,并增加微管蛋白纯度。

Determination of BET Protein Binding Affinities to I-BET726: For determination of binding affinities to BET protein bromodomains, I-BET726 is titrated against truncates containing both BD1 and BD2 of BRD2 (10 nM), BRD3 (10 nM), and BRD4 (10 nM) in 50 mM HEPES pH7.5, 150 mM NaCl, 5% Glycerol, 1 mM DTT and 1 mM CHAPS in the presence of an Alexa 647 derivative (50 nM) of fluorescent ligand. After equilibrating for 1 h, the bromodomain protein: ligand interaction is detected using Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) following the addition of 1.5 nM europium chelate labeled anti-6His antibody. Plates are read using an Envision Plate reader (λEX = 337 nm, λEM = 615 nm, λEM = 665 nm; dual dichroic = 400 nm & 630 nm). These data are fitted to a four parameter IC50 model using Graphit data analysis software.

103476-89-7

C40H78N12O12S

951.2

亮肽素

DMSO:88 mg/mL (185 mM)
Ethanol:88 mg/mL (185 mM)
H2O:87 mg/mL (182.9 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years