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Losartan potassium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Losartan potassium图片
CAS NO:124750-99-8
包装与价格:
包装价格(元)
500 mg电议
1 g电议
1 mL*10 mM(in DMSO)电议

产品名称
DuP-753 potassium
DuP 753
MK 954
洛沙坦钾
氯沙坦钾
产品介绍
Losartan potassium 是一种血管紧张素 II 受体 1 型 (AT1)拮抗剂,能够与血管紧张素 II 与 AT1 的结合竞争,其IC50=20 nM。

产品描述

Losartan, an Angiotensin II Receptor Blocker, selectively and competitively blocks the binding of angiotensin II to the angiotensin I (AT1) receptor.

体外活性

Losartan competes with the binding of angiotensin II to AT1 receptors. The concentration that inhibits 50% of the binding of angiotensin II (IC50) is 20 nM[1]. Losartan (40?μM) affects ISC but prevents the effect of ANGII on ISC[2]. Losartan significantly reduces Ang II-mediated cell proliferation in endometrial cancer cells. The combination of losartan and anti-miR-155 has a significantly greater antiproliferative effect compared to each drug alone[3].

细胞实验

An MTT assay is used to measure cell proliferation and viability. For the assay, 5000 cells in 200?μL media per well are seeded in a 96 well plate. After overnight incubation to allow for cell attachment, the medium is removed by suction. MTT at 1?mg/mL concentration in serum-free medium is added and then incubated for 4?h at 37°C. After removal of MTT solution, 100?μL of DMSO is added to dissolve formazan crystals. Absorbance at 570?nm and at 600?nm as a reference is then measured using a microplate reader. The difference in absorbance is thus relative to the extent of cell survival.

Cas No.

124750-99-8

分子式

C22H22ClKN6O

分子量

461.01

别名

DuP-753 potassium;DuP 753;MK 954;洛沙坦钾;氯沙坦钾

储存和溶解度

DMSO:46.1 mg/mL (100 mM)
H2O:46.1 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years