BW-A78U 抑制 PDE4，其IC50值为 3 μM。

BW-A 78U is a PDE4 inhibitor (IC50: 3 μM). It is not inhibition on the lipopolysaccharide (LPS)-induced TNF-α release.

Mononuclear cells were incubated for 30 min with BW-A 78U (0.01-10 μM). The cells were then stimulated with lipopolysaccharide (10 μg/mL) overnight at 37°C in an atmosphere of 5% CO2 at 100% humidity. Cell-free supernatants were collected, centrifuged (2000 g), and stored frozen at -20°C before TNF-α determination. TNF-α concentrations in cell culture supernatants were determined by specific ELISA using a commercial kit. The sensitivity of the assay was 1 pg/mL. The absorbance at 450 nm was assessed with an ELISA reader.

101155-02-6

C13H12FN5

257.272

BW-A78U

DMSO:100 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years