IBMX 是广谱的磷酸二酯酶抑制剂，对PDE3，PDE4和PDE5 的IC50值分别为 6.5，26.3 和 31.7 μM。

IBMX is a pan-phosphodiesterase (PDE) inhibitor (IC50s: 6.5/26.3/31.7 μM for PDE3/PDE4/PDE5).

The IC50 values of IBMX for PDE3, 4 and 5 were 6.5, 26.3 and 31.7 μM, respectively [1]. IBMX (100 μM) activated ROMK channels and prevented further channel activation by ANG II or cGMP [2].

In comparison with the control group, IBMX significantly increased plasma glucose (blood glucose, mg/dl, control= 141, IBMX= 210) [3]. On days 1, 2, and 3, IBMX did not significantly attenuate the cocaineinduced increase in activity. However, beginning on the fourth day of the study, the activity of rats in the IBMX/cocaine groups was significantly lower than the activity of animals that received cocaine but no IBMX (saline/cocaine). The activity of animals in the IBMX/saline group was compared with the activity of animals from a preliminary study that received i.c.v. saline, followed by i.p. saline. This comparison revealed that i.c.v. IBMX had no effect on the basal levels of activity [4].

Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits [1].

Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated [3].

28822-58-4

C10H14N4O2

222.248

1-Methyl-3-Isobutylxanthine;IBMX,3-异丁基-1-甲基黄嘌呤;Methylisobutylxanthine;3-Isobutyl-1-methylxanthine;Isobutylmethylxanthine

DMSO:16.7 mg/mL(75 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years