Evans Blue 是一种突触泡谷氨酸吸收的有效抑制剂，同时也是一种AMPA/kainate受体拮抗剂。

Evans blue is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).

Evans Blue is found to be inhibiting DNA binding of NF-κB at a low concentration of 100 μM[1]. It has proven over the years to be a dependable stain for microscopic determination of cell death[2]. Evans Blue is also a known blocker of a subset of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptors (IC50=355 nM) for the subunit combination GluR1,2[5]. Evans blue is the first known δ-subunit-specific antagonist of ENaC and activates large-conductance Ca2+-activated K+ channels in sheep bladder myocytes and cultured endothelial cells of human umbilical veins[5].

Evans blue pretreatment could inhibit the mast cells degranulation and that may be the main mechanism for prevent animals from C48/80-triggered anaphylaxis and systemic inflammation[4]. Evans blue modulates the non-N-methyl-d-aspartate receptor in rat thalamic neurons, blocks the P2X-purinergic receptor in rat vas deferens, and inhibits the glutamate transporter in rat brain synaptic vesicles[6].

314-13-6

C34H24N6Na4O14S4

960.79

C.I. 23860;Direct Blue 53;伊文思蓝

H2O:104.1 mM
DMSO:104.1 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years