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BAM7
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:331244-89-4
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
50 mg电议

产品介绍
BAM7 是一种促凋亡 Bax 的直接和特异性激活剂,IC50为 3.3 μM。

产品描述

BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).

体内活性

体外实验中,BAM7触发BAX-介导的孔形成,BAX低聚化及BAX-依赖的细胞死亡。通过触发细胞内BAX激活,BAM7可选择性诱导BAX-介导的细胞凋亡。BAM7只对含BAX的细胞系有杀灭效果,产生BAX-介导的细胞凋亡的生化和形态特征。BAM7可与BAX的N-末端的BH3结构结合沟直接结合。BAM7与BAX直接在表面相互作用,经BIM BH3螺旋触发BAX激活。BAM7剂量和时间性地触发BAX从单体到低聚物的转换。

激酶实验

Fluorescence polarization binding assays: Direct binding curves are first generated by incubating FITC-BIM SAHB (50 nM) with serial dilutions of fulllength BAX, BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC or BAKΔC and fluorescence polarization measured at 20 minutes on a SpectraMax M5 microplate reader. For competition assays, a serial dilution of small molecule or acetylated BIM SAHB (Ac-BIM SAHB) is combined with FITC-BIM SAHB (50 nM), followed by the addition of recombinant protein at ~EC75 concentration, as determined by the direct binding assay (BAX, BAKΔC: 500 nM; BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC: 200 nM). Fluorescence polarization is measured at 20 minutes and IC50 values calculated by nonlinear regression analysis of competitive binding curves using Prism software.

细胞实验

MEFs (2.5 × 103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7, ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37 °C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent according to the manufacturer's protocol, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells.(Only for Reference)

Cas No.

331244-89-4

分子式

C21H19N5O2S

分子量

405.47

储存和溶解度

DMSO:4.1 mg/mL (10 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years