Cortisone acetate 是 Cortisol（一种糖皮质激素）的氧化代谢产物。它在高浓度下能够部分干预 Glucocorticoid 与Glucocorticoid-receptor的结合。它具有免疫抑制和消炎的作用。

Cortisone Acetate is the acetate salt form of cortisone, a synthetic or semisynthetic analog of the naturally occurring cortisone hormone produced by the adrenal glands with anti-inflammatory and immunomodulating properties. Cortisone acetate diffuses through the cell membrane and binds to nuclear glucocorticoid receptors. The receptor-ligand complex binds to promotor regions of certain genes and initiates RNA transcription. This results in an induction of synthesis of certain anti-inflammatory proteins while inhibiting the synthesis of certain inflammatory mediators.

Levels of enzymatically active caspase-3 are quantified using the ApoAlert Caspase Fluorescent assay kit. Briefly, 1×106 BC-1 PEL cells are treated with 50 μM Miltefosine, 50 μM Perifosine, or 20 nM NVP-BEZ235, as well as the respective vehicle controls. Cells are harvested and lysed 12 hours later. Equivalent micrograms of cell lysate for all samples are incubated with a fluorogenic caspase-3 substrate (DEVD-AFC). Cleavage of DEVD by caspase-3 releases AFC, the fluorescence of which is measured using a FLUOstar OPTIMA fluorometer, with excitation and emission filter wavelengths set to 400 and 505 nm, respectively[3].

50-04-4

C23H30O6

402.49

Cortisone 21-acetate;醋酸可的松;NSC 49420

Ethanol:<1 mgml
DMSO:7 mg/mL (17.39 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years