Kartogenin 是一种人间充质干细胞分化为软骨细胞的诱导剂(EC50:100 nM)。它能够结合纤维蛋白A，破坏其与转录因子核心结合因子 β 亚基 (CBFβ) 的相互作用，并通过调节 CBFβ-RUNX1 转录程序，诱导软骨形成，可用于研究骨关节炎。

Kartogenin is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.

100 nM Kartogenin会刺激小鼠真皮中胶原蛋白合成.此外,Kartogenin刺激小鼠肢体骨骼生长和滑液关节发育.10 μM 和100 μM Kartogenin分别促进胶原酶VII诱导的和手术诱导的小鼠OA模型的软骨修复.

Kartogenin通过激活smad4/smad5途径促进真皮成纤维细胞的I型胶原合成,对成纤维细胞的活性、形态和存活没有明显细胞毒性。Kartogenin通过增强CBFβ细胞核定位,诱导hMSCs向软骨细胞分化,并保护关节软骨细胞。

For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer's instructions, cell viability and viability are assessed by AlamarBlue assay.(Only for Reference)

4727-31-5

C20H15NO3

317.344

KGN

DMSO:63 mg/mL (198.52 mM)
Ethanol:18 mg/mL (56.72 mM),warmed
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years