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iCRT3
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
iCRT3图片
CAS NO:901751-47-1
包装与价格:
包装价格(元)
2 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
1 mL*10 mM(in DMSO)电议

产品介绍
iCRT3 是一种Wnt和 β-连环蛋白应答转录抑制剂。

产品描述

iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.

体外活性

Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively.

体内活性

The IL-6 levels in the 10?mg/kg iCRT3 treatment mice are lower.

激酶实验

In the determination of full-length baculovirus expressed Syk kinase activity, the reaction system was 25 μL containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 2 mM DTT, 0.1 mM Na3VO4, 10 mM MgCl2, 0.5 μM Promega PTK Biotin Peptide Substrate 1, 0.01% casein, 1, 0.01% Triton X-100, 0.25% Glycerol, and 40 mM ATP (Km for ATP). After incubation for 60 minutes at room temperature, the reaction was stopped by adding 30 mM EDTA (30 μL of SA-APC and 4 nM PT-66 antibody). After measuring the plate, the IC50 value of the test compound was calculated using a 4-parameter linear regression algorithm.

细胞实验

Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively.

动物实验

NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly.

Cas No.

901751-47-1

分子式

C23H26N2O2S

分子量

394.53

储存和溶解度

DMSO:150 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years