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IM-12
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
IM-12图片
CAS NO:1129669-05-1
包装与价格:
包装价格(元)
10 mg电议
50 mg电议
100 mg电议

产品名称
IM 12
产品介绍
IM12 是一种GSK-3β的抑制剂,其 IC50=53 nM,可提高 Wnt 信号传导。

产品描述

IM-12, an effective GSK-3β inhibitor(IC50=53 nM), regulates Wnt signalling.

体内活性

在hNPCs中(IC50=3.8 μM),IM-12能够抑制GSK-3β活性,增加β-连环蛋白浓度。在人神经祖细胞中,IM-12通过对于Wnt信号通路的激活,促进细胞增殖。

激酶实验

GSK-3β activity assay: IC50 of new synthesised compound IM-12 to GSK-3β is determined by a luminometric GSK-3β activity assay. Briefly, compounds are tested in different concentrations diluted in assay buffer containing final concentrations of: 4 mM MOPS pH 7.2; 0.4 mM EDTA; 1 mM EGTA; 2.5 mM β-glycerophosphate; 4 mM MgCl2; 40 μM BSA; 0.05 mM DTT. Four microlitres of diluted compounds are added to 25 μM pGS-2 peptide substrate, 20 ng recombinant GSK-3β and 1 μM ATP to a total assay volume of 40 μl. The enzymatic reaction is stopped after 30 min of incubation at 30 °C by adding 40 μl KinaseGlo. The luminometric signal is allowed to stabilise for 10 min and the measured with a Glomax? 96 Microplate Luminometer.

细胞实验

To measure viable cells, 50-100 μL of cell suspension is analyzed using CASY technology with the appropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Then the medium is changed to proliferation medium with added substances at indicated concentrations. The cell number was determined every 24 h. Cells were exposed to the added drugs during the whole experiment, whereas the media is changed every 24 h.(Only for Reference)

Cas No.

1129669-05-1

分子式

C22H20FN3O2

分子量

377.41

别名

IM 12

储存和溶解度

DMSO:70 mg/mL (185.5 mM)
Ethanol:10 mg/mL (26.49 mM),warmed
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years