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HO-3867
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
HO-3867图片
CAS NO:1172133-28-6
包装与价格:
包装价格(元)
1 mg电议
2 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
HO3867
产品介绍
HO3867是一种STAT3的选择性有效抑制剂,有抗肿瘤活性。

产品描述

HO-3867, an analog of curcumin, is a sspecific STAT3 inhibitor.

激酶实验

Scintillation Proximity Assay, 125I-FGF-2 Binding: SPA protein A beads are supplied as a suspension in PBS at 20 mg/mL, then diluted with binding buffer (KCl, 400 mg/L; MgSO4 200 mg/L; NaCl 6.4 g/L; NaHCO3 3.7 g/L; NaH2PO4 0.141 mg/mL; bis Tris Propane 11.292 g/L; Glucose 4.5 g/L; Gelatin 0.1 %; pH 7.0) at 10 mg/mL. 125I-FGF-2 radioligand and FGFR-1IIIc? - Fc Chimera are diluted into binding buffer. Binding was performed on 96-well plates coated with 0.1 % gelatin. Total assay volume is 0.1 mL. Binding of 125I-FGF-2 is determined by incubation of SPA beads coated with protein A (0.5 mg/assay) with FGFR-1IIIc? - Fc chimera soluble receptor (5 ng/assay), FGF-2 (20 ng/assay) is used for non-specific binding determinations.

细胞实验

Cell viability is determined by a colorimetric assay using MTT. In the mitochondria of living cells, yellow MTT undergoes a reductive conversion to formazan, producing a purple color. Cells, grown to ~80% confluence in 75-mm flasks, are trypsinized, counted, seeded in 96-well plates with an average population of 7,000 cells/well, incubated overnight, and then treated with HO-3867 for 24 h. All experiments are done using 8 replicates and repeated at least three times. (Only for Reference)

Cas No.

1172133-28-6

分子式

C28H30F2N2O2

分子量

464.557

别名

HO3867

储存和溶解度

Ethanol:6 mg/mL (12.91 mM),warmed
H2O:<1 mgml
DMSO:11 mg/mL (23.7 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years