您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > Acetylcysteine
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Acetylcysteine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Acetylcysteine图片
CAS NO:616-91-1
包装与价格:
包装价格(元)
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
NAC
乙酰半胱氨酸
N-Acetyl-L-cysteine
LNAC
易咳净
产品介绍
Acetylcysteine 是粘液溶解剂,可用于减少粘液的厚度。它是ROS抑制剂,可诱导细胞凋亡,并具有抗流感病毒活性。它是半胱氨酸前体,通过中和花生四烯酸依赖的5-脂氧合酶活性所产生的毒性脂质来防止血红素诱导的铁中毒。

产品描述

Acetylcysteine is the N-acetyl derivative of CYSTEINE. It is used as a mucolytic agent to reduce the viscosity of mucous secretions. It has also been shown to have antiviral effects in patients with HIV due to inhibition of viral stimulation by reactive oxygen intermediates.

体外活性

N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents the death of PC12 cells and sympathetic neurons[2]. N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by the withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that N-acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[3].

体内活性

N-acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group N-acetylcysteine 300. Lung glutathione peroxidase is significantly increased in group N-acetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences[1].

细胞实验

For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

动物实验

Rats are randomly allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given N-acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group N-acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group N-acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group N-acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.[1]

Cas No.

616-91-1

分子式

C5H9NO3S

分子量

163.19

别名

NAC;乙酰半胱氨酸;N-Acetyl-L-cysteine;LNAC;易咳净

储存和溶解度

DMSO:31 mg/mL (190 mM)
Ethanol:31 mg/mL (190 mM)
H2O:30 mg/mL (183.8 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years