Nisoldipine 是一种高效特异的L型Cav1.2通道阻断剂，IC50=10 nM。

Nisoldipine is a dihydropyridine calcium channel antagonist that acts as a potent arterial vasodilator and antihypertensive agent.

对于Timothy综合征病人,Nisoldipine（IC50=267 nM）通过抑制钙离子从L型钙通道流入,降低动脉平滑肌收缩性及随后的血管收缩 , 导致血管舒张,且降低血压.

在豚鼠心室肌细胞中,Nisoldipine延迟整流K+通道,快速激活延迟整流K+通道抑制IKr(IC50=23 μM), 及缓慢激活延迟整流K+通道抑制IKs(IC50=40 μM)。

Binding experiments of electrophysiology: CHO cells expressing the subunit of the voltage-dependent L-type Ca2+ channel are cultrured in medium without serum in the presence of different concentrations of Nisoldipine. Then Ca2+ channel current elicited from a holding potential of -100 mV or -50 mV is recorded at room temperature with the whole-cell configuration of the patch-clamp method using the List EPC-7 patch-clamp amplifer and pClamp software. The concentration of competitor inhibiting 50% of the specific binding represents IC50.

The myocytes are bathed in normal Tyrode's solution, held at -80 mV, and depolarised after 200-ms prepulses (-40mV) to more positive potentials for 500 ms at 0.1 Hz, tail currents are recorded on repolarisations to -40mV. The myocytes are exposed to 10-100 mM Nisoldipine for 8-10 minutes. Then the whole-cell membrane currents are recorded using an EPC-7 amplifier.(Only for Reference)

63675-72-9

C20H24N2O6

388.42

BAY-k 5552;尼索地平

H2O:<1 mgml
Ethanol:56 mg/mL (144.2 mM)
DMSO:71 mg/mL (182.8 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years