Daidzein 是一种来自大豆的异黄酮提取物,是酪氨酸激酶抑制剂染料木黄酮的无活性类似物,具有抗氧化和植物雌激素特性。
产品描述
Daidzein is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.
体内活性
Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. Daidzein results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats. [5]
细胞实验
Daidzein is dissolved in DMSO and then diluted with appropriate media[1]. HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid[1].
Cas No.
486-66-8
分子式
C15H10O4
分子量
254.241
别名
大豆素;Isoflavone;黄豆苷元
储存和溶解度
DMSO:25.4 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years