您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > Warangalone
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Warangalone
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Warangalone图片
CAS NO:4449-55-2
包装与价格:
包装价格(元)
5 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
攀登鱼藤异黄酮
Scandenolone
产品介绍

产品描述

Warangalone is an anti-malarial compound that can inhibit the growth of both strains of parasite 3D7 (chloroquine-sensitive) and K1 (chloroquine-resistant) with IC50s of 4.8 μg/mL and 3.7 μg/mL, respectively.

体外活性

Warangalone can also inhibit cyclic AMP-dependent protein kinase catalytic subunit (cAK) with an IC50 of 3.5 μM [2]. When HL-60 cells are exposed to Warangalone (30 μM) for 24 h, Warangalone induces a significant decrease (8%) in cell viability compared to controls. Warangalone also inhibits HL-60 cell growth within 24 h in a time-dependent fashion. A time-dependent increase in caspase-9 activity is observed in Warangalone-treated cells [3].

激酶实验

Cells are seeded in 24-well plates at a density of 3×10^6 cells per well. After exposure of the cells to Warangalone for the allotted time periods, the cells are washed three times with PBS and then lysed in a lysis buffer for 10 min on ice. The protein content of the cell lysates is assayed with a Micro BCA reagent. Cell lysates containing 50 μg of protein are incubated with a caspase-3 fluorogenic substrate (DEVD-AFC) or a caspase-9 fluorogenic substrate (LEHD-AFC) for 1 h at 37°C. Caspase activity is measured by fluorometric detection [3].

细胞实验

Cells are seeded in 96-well plates at a density of 1×10^5 cells per well. The cells are maintained for 24 h at 37°C and then Warangalone (30 μM) is added to the culture medium. MTS solution is added to the 96-well plates at the indicated time points, and the cells are incubated for 1 h at 37°C. The absorbance is measured at a wavelength of 490 nm with a microplate counter [3].

Cas No.

4449-55-2

分子式

C25H24O5

分子量

404.46

别名

攀登鱼藤异黄酮;Scandenolone

储存和溶解度

DMSO:Soluble
Powder: -20°C for 3 years
In solvent: -80°C for 2 years