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Asaraldehyde
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Asaraldehyde图片
CAS NO:4460-86-0
包装与价格:
包装价格(元)
20 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
细辛醛
Asaronaldehyde
Asarylaldehyde
2,4,5-trimethoxy-Benzaldehyde
产品介绍
Asarylaldehyde 是COX-2抑制剂,能够明显降低环加氧酶 II (COX-2) 的活性(IC50:100 μg/mL)。

产品描述

Asaraldehyde, a natural COX-2 inhibitor, exhibits 17-fold selectivity than COX-1.

体内活性

Asaraldehyde(100 μg/mL)对COX-2的抑制效果更高。Asaraldehyde(100 μg/mL)对前列腺素H内过氧化物合成酶-1的活性有轻微抑制作用(3.32%),对前列腺素H内过氧化物合成酶-2的活性有一定抑制效果(52.69%)。Asaraldehyde可使C/EBPβ, C/EBPδ和C/EBPα下调,对PPARγ1、PPARγ2以及乙酰辅酶A羧化酶的表达有抑制作用。

激酶实验

Hedgehog cell assay: This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO2. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO2. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack

细胞实验

3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes.(Only for Reference)

Cas No.

4460-86-0

分子式

C10H12O4

分子量

196.2

别名

细辛醛;Asaronaldehyde;Asarylaldehyde;2,4,5-trimethoxy-Benzaldehyde

储存和溶解度

DMSO:39 mg/mL (198.77 mM)
Ethanol:16 mg/mL (81.54 mM)
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years