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Psoralen
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Psoralen图片
CAS NO:66-97-7
包装与价格:
包装价格(元)
20 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
psoralene
Ficusin
Furocoumarin
补骨脂素
产品介绍
Psoralen是从补骨脂种子中提取的一种香豆素。它具有如抗癌、抗氧化、抗抑郁、抗癌、抗菌、抗病毒等多种生物学特性。

产品描述

Psoralen is a furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.

体外活性

Psoralen inhibits the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. Psoralen reverses MDR(multidrug resistance) through inhibiting ATPase activity rather than reducing P-gp expression. Psoralen inhibits the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-κB. Psoralens are photoactive compounds that readily alkylate DNA when activated by longwave ultraviolet light. Proliferation has been significantly promoted in MCF-7/ADR cells treated with low concentration of psoralen (<10.75 μM) and inhibited with high concentration(>21.5 μM). Psoralen can inhibit metastasis of breast cancer. Psoralen mediates a variety of cell processes including cell death, proliferation, inflammation and migration[1].

体内活性

Psoralen has been characterized as a tumor suppressor in various tumors[1]. Psoralen ameliorates sex hormone deficiency-induced osteoporosis in female and male mice. It has antiosteoporosis effect in ovariectomy-induced osteoporotic rats via stimulating osteoblastic differentiation from bone mesenchymal stem cells[2].

细胞实验

The effects of psoralen on cell proliferation are measured by MTT assay. MCF-10A and MCF-7/ADR cells are cultured in 96-well plates at a cell density of 2×104 cells per well for 48 h. The medium is then removed and replaced by fresh medium containing different concentrations of psoralen (0, 21.5, 43.0, 64.5, 86.0, 107.5 μM) for 48 h. Cells in the negative control group are incubated with RPMI-1640 culture medium supplemented with 0.1% dimethyl sulfoxide (DMSO). Cells are incubated with 10 µL MTT (5 mg/mL) for 4 h, and then discarded the medium and added 200 µL DMSO. The spectrophotometric absorbance is measured at 490 nm with enzyme-labeling instrument after the crystals were fully dissolved. (Only for Reference)

Cas No.

66-97-7

分子式

C11H6O3

分子量

186.166

别名

psoralene;Ficusin;Furocoumarin;补骨脂素

储存和溶解度

Ethanol:<1 mgml
H2O:<1 mgml
DMSO:35 mg/mL (188 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years