Dioscin 是一种来源植物的天然甾体皂苷，针对多种癌细胞有较好的抗癌活性。

Dioscin is a saponin with antitumor activities.

小鼠LD50:>300 mg/kg

Dioscin通过下调Hela细胞中caspase-8的活性,并上调caspase-9的活性诱导细胞凋亡。Dioscin抑制MDA-MB-435,H14,HL60和HeLa细胞系的生长,IC50分别为2.6,0.8,7.5以及4.5 μM。

Rat liver cytosol is prepared from a separate piece of liver from rat. Assays are carried out in a total volume of 0.1 mL containing 10 mg of cytosolic protein, and components according to Rilling. All reaction components (except IPP) are mixed and kept on ice for 15 min. Reactions are initiated by the addition of [14C]IPP and incubation at 37°C. Reactions are stopped after 5 min by addition of 0.4 mL MeOH/HCl (4/1, by vol.) and the samples are incubated a further 15 min to hydrolyze the allylic pyrophosphates to petroleum ether-extractable products. Following addition of 0.5 mL water and 1 mL petroleum ether, 50% of the upper (petroleum ether-extractable) phase is taken for liquid scintillation analysis. Preliminary experiments indicated that the reaction is linear with time and protein under these conditions, and no more than 10% of the substrate is consumed.

19057-60-4

C45H72O16

869.055

Saponin;Collettiside III;薯蓣皂苷;薯蓣皂甙;CCRIS 4123;山药

Ethanol:22 mg/mL (25.31 mM)
DMSO:100 mg/mL (115.06 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years