SYBR GREEN 1 是一种不对称花青染料，用于检测核酸。它由一个 N-烷基化的苯并噻唑或苯并恶唑环系统组成，通过一甲基桥连接到吡啶鎓或喹啉鎓环系统。 SYBR Green I 与 dsDNA 的小沟结合，在 480 nm 波长处被激发。它具有 520 nm 的峰值荧光发射。

SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Precasting SYBR Green I Gels:Precast nondenaturing polyacrylamide or agarose gels with SYBR Green I staining solution, which is diluted from the SYBR Green I stock reagent 1:10,000 prior to pouring the gel. The DNA detection limit for gels precast with SYBR Green I stain may be slightly higher (on the order of 30 to 40 pg/band) than for gels stained after electrophoresis (less than 20 pg/band), and the migration rate of DNA fragments in SYBR Green I gels may be significantly slower than the migration rate of the same fragments in a gel containing no dye.

163795-75-3

SYBRGREENⅠ核苷酸胶体染料;SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSO;SUPER Green I （效果同SYBR Green I）核酸染料（10,000× DMSO溶液）（电泳级）

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years