| 包装 | 价格(元) |
| 100mg | 电议 |
| 250mg | 电议 |
| 500mg | 电议 |
Cell lines | Human fibroblasts |
Preparation Method | Human fibroblasts were incubated for 72 h with low (50 μM) and high (200 μM) concentrations of large, unilamellar (LUV) soya-phosphatidylcholines liposomes in the presence and absence of vitamin E (soya-phosphatidylcholines:vitamin E; 10:0.5). |
Reaction Conditions | 50 μM and 200 μM, 72h |
Applications | No significant changes in cell viability were observed between untreated fibroblasts and those incubated with 50 μM soya-phosphatidylcholines liposomes. In contrast, a marked loss of cells was apparent at a concentration of 200 μM soya-phosphatidylcholines liposomes which was reversible by the addition of vitamin E. |
Animal models | Male Wistar albino rats (200-250 g) |
Preparation Method | Treatment of soya phosphatidylcholines (5 mg/kg/d and 10 mg/kg/d, i.p.) and dexamethasone (2.5 mg/kg/d, i.p.) were started from the 28th day of study till the end of the study in respective groups. At the end of the experiment, lung function parameters, total and differential leukocytes count in blood and BALf; inflammatory cytokines (IL-4, IL-5, and IL-13) and metabolic parameters (glucose, cholesterol, and triglyceride) in serum were measured in all the rats. Histopathological examination of lungs was performed in all rats. |
Dosage form | 5 mg/kg/d and 10 mg/kg/d, i.p. |
Applications | The results displayed lung function parameters, total and differential leukocytes count in blood and BALf; inflammatory cytokines (IL-4, IL-5, and IL-13) and triglyceride shown significantly decreased, but serum glucose and cholesterol level has no change. |
| 产品描述 | Phosphatidylcholines,soya is an essential phospholipid from soybean for cell formation, growth, and death.[1]It is always used in the preparation of liposomes.[2]Soya lecithin origin phosphatidylcholine (Soya PC) was identified as a natural TLR4 antagonist by the computational study.[3] In vitro, fibroblasts display sensitivity toward the soybean phosphatidylcholine liposomes with an IC50of 150 μM. Because of the lesser efficency with these liposomes bind to cells, in vitro experiment it shown that phosphatidylcholine liposomes produced no detectable growth inhibitory effects (IC50> 400 lM) . soya-phosphatidylcholines liposomes present in abundance linoleic acid, which as an essential fatty acid normally obtained from external sources, resulting in the proliferation of the broblasts seen at concentrations below 50 μM and the reduction in cell proliferation occurring at soya-phosphatidylcholines phospholipid concentrations above 50 μM.[2]In vitro, treatment with 10 and 50 μM soya-phosphatidylcholines liposomes, there exsits an increase in the number of fibroblasts per well comparedto the control group, while at phospholipid concentrations exceeding 100μM, a clear inhibitory effect was observed.[4] In vivo experiment it demonstrated that treatment with 5 mg/kg and 10 mg/kg Soya phosphatidylcholines significantly decreased the respiration rate whereas, tidal volume and airflow rate were found to be significantly increased compared to asthmatic rats. In the meanwhile, the total leukocytes and differential leukocytes count, eosinophils, lymphocytes in the blood of asthmatic rats also showed significantly decreased after treatment with 5 mg/kg and 10 mg/kg Soya PC. In vivo test it indicated that serum triglyceride level was obviously decreased in soya phosphatidylcholines (5 mg/kg and 10 mg/kg) treated asthmatic rats, however, treatment with 5 mg/kg and 10 mg/kg Soya phosphatidylcholines did not show the modulation in serum glucose and cholesterol level in asthmatic rats. References: |
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