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Phosphatidylcholines,soya
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
磷脂酰胆碱,大豆是一种来自大豆的磷脂酰胆碱,用于制备脂质体。

Cell lines

Human fibroblasts

Preparation Method

Human fibroblasts were incubated for 72 h with low (50 μM) and high (200 μM) concentrations of large, unilamellar (LUV) soya-phosphatidylcholines liposomes in the presence and absence of vitamin E (soya-phosphatidylcholines:vitamin E; 10:0.5).

Reaction Conditions

50 μM and 200 μM, 72h

Applications

No significant changes in cell viability were observed between untreated fibroblasts and those incubated with 50 μM soya-phosphatidylcholines liposomes. In contrast, a marked loss of cells was apparent at a concentration of 200 μM soya-phosphatidylcholines liposomes which was reversible by the addition of vitamin E.

Animal models

Male Wistar albino rats (200-250 g)

Preparation Method

Treatment of soya phosphatidylcholines (5 mg/kg/d and 10 mg/kg/d, i.p.) and dexamethasone (2.5 mg/kg/d, i.p.) were started from the 28th day of study till the end of the study in respective groups. At the end of the experiment, lung function parameters, total and differential leukocytes count in blood and BALf; inflammatory cytokines (IL-4, IL-5, and IL-13) and metabolic parameters (glucose, cholesterol, and triglyceride) in serum were measured in all the rats. Histopathological examination of lungs was performed in all rats.

Dosage form

5 mg/kg/d and 10 mg/kg/d, i.p.

Applications

The results displayed lung function parameters, total and differential leukocytes count in blood and BALf; inflammatory cytokines (IL-4, IL-5, and IL-13) and triglyceride shown significantly decreased, but serum glucose and cholesterol level has no change.

产品描述

Phosphatidylcholines,soya is an essential phospholipid from soybean for cell formation, growth, and death.[1]It is always used in the preparation of liposomes.[2]Soya lecithin origin phosphatidylcholine (Soya PC) was identified as a natural TLR4 antagonist by the computational study.[3]

In vitro, fibroblasts display sensitivity toward the soybean phosphatidylcholine liposomes with an IC50of 150 μM. Because of the lesser efficency with these liposomes bind to cells, in vitro experiment it shown that phosphatidylcholine liposomes produced no detectable growth inhibitory effects (IC50> 400 lM) . soya-phosphatidylcholines liposomes present in abundance linoleic acid, which as an essential fatty acid normally obtained from external sources, resulting in the proliferation of the broblasts seen at concentrations below 50 μM and the reduction in cell proliferation occurring at soya-phosphatidylcholines phospholipid concentrations above 50 μM.[2]In vitro, treatment with 10 and 50 μM soya-phosphatidylcholines liposomes, there exsits an increase in the number of fibroblasts per well comparedto the control group, while at phospholipid concentrations exceeding 100μM, a clear inhibitory effect was observed.[4]

In vivo experiment it demonstrated that treatment with 5 mg/kg and 10 mg/kg Soya phosphatidylcholines significantly decreased the respiration rate whereas, tidal volume and airflow rate were found to be significantly increased compared to asthmatic rats. In the meanwhile, the total leukocytes and differential leukocytes count, eosinophils, lymphocytes in the blood of asthmatic rats also showed significantly decreased after treatment with 5 mg/kg and 10 mg/kg Soya PC. In vivo test it indicated that serum triglyceride level was obviously decreased in soya phosphatidylcholines (5 mg/kg and 10 mg/kg) treated asthmatic rats, however, treatment with 5 mg/kg and 10 mg/kg Soya phosphatidylcholines did not show the modulation in serum glucose and cholesterol level in asthmatic rats.

References:
[1]. Chiang YP, et al. Sphingomyelin synthases 1 and 2 exhibit phosphatidylcholine phospholipase C activity. J Biol Chem. 2021 Dec;297(6):101398.
[2]. Berrocal MC, et al. Comparison of the effects of dimyristoyl and soya phosphatidylcholine liposomes on human fibroblasts. Drug Deliv. 2000 Jan-Mar;7(1):37-44.
[3]. Thakur VR, et al. An anti-asthmatic activity of natural Toll-like receptor-4 antagonist in OVA-LPS-induced asthmatic rats. Clin Exp Pharmacol Physiol. 2018 Nov;45(11):1187-1197.
[4]. Berrocal MC, et al. Aging of soya-PC liposomes containing vitamin E reverses the stimulating effects of freshly prepared liposomes on growth of fibroblasts in culture. Drug Deliv. 1998;5(4):243-50.