ADHP 是荧光过氧化物酶底物，其λex=530 nm，λem=590 nm。

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6/s. The kobs also increase over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second-order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.

119171-73-2

C14H11NO4

257.24

10-乙酰基-3,7-二羟基吩嗪;10-Acetyl-3,7-dihydroxyphenoxazine

DMSO:60 mg/ml (233.25 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years