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Protopine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Protopine图片
CAS NO:130-86-9
包装与价格:
包装价格(元)
20 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
Corydinine
Biflorine
原阿片碱
Hypercorine
Macleyine
Fumarine
产品介绍
Protopine 是一种常存在于亚洲东北部的一些植物中的异喹啉类生物碱,常用于抗肿瘤、抗炎活性的研究。

产品描述

Protopine is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic.

激酶实验

HT1080 cells are seeded in 24-well plates (2×104 cells per well) and treated with Dorsomorphin in the presence or absence of glucose or 10 mM 2DG for 2 h. HT1080 cells that overexpressed the wild-type and dominant negative AMPKα1 are prepared by transfecting plasmid DNA (pAMPKα1-wt, pAMPKα1-D168A and pcFlag as a control) in 6-well plates, seeding in 24-well plate and treating with UPR inhibitors. Cells are lysed with cell lysis buffer (20 mM Tris-HCl, pH 7.5, 250 mM NaCl, 10% glycerol, 0.5% NP-40, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1 μg/mL pepstatin, 0.5 μg/mL leupeptin, 5 mM NaF, 2 mM Na3Vo4, 2 mM β-glycerophosphate, 1 mM DTT). Relative AMPK kinase activity (mean±SD of duplicate determinations) to control sample (vehicle or pcFlag under normal growth conditions) is determined using the CycLex AMPK kinase assay kit[2].

Cas No.

130-86-9

分子式

C20H19NO5

分子量

353.37

别名

Corydinine;Biflorine;原阿片碱;Hypercorine;Macleyine;Fumarine

储存和溶解度

DMSO:28 mg/mL(79.2 mM)
Chloroform 1:15:Soluble
Powder: -20°C for 3 years
In solvent: -80°C for 2 years