Ciclesonide 是一种糖皮质激素,具有强效抗炎活性,可用于研究哮喘。
产品描述
Ciclesonide(RPR251526) is a glucocorticoid used for the treatment of obstructive airway diseases.
体外活性
Ciclesonide is effectively taken up into human alveolar type II epithelial (A549) cells and metabolized to its pharmacologically active metabolite des-CIC and fatty acid conjugates of desCIC[2].
细胞实验
Metabolism of ciclesonide: After initial cultivation, A549 cells are washed five times with 10 ml/dish sterile PBS. An ethanolic stock solution of ciclesonide is diluted 1000-fold with assay medium to generate the initial ciclesonide concentration. The cells are incubated for 1 h in DMEM/0.1% (w/v) BSA (5 ml/dish) containing 0.02 μM ciclesonide as described above in a CO2 incubator. At the end of the incubation period, dishes are washed five times with PBS and incubated for 3, 6, or 24 h at the same conditions in 5 ml/dish fresh medium (DMEM/0.1% (w/v) BSA). At each indicated time point, the medium is removed by aspiration, 5 mM EDTA-4Na/PBS added and dishes are incubated for further 5 min. The cells are detached from the dishes and harvested. Each dish is washed five times with icechilled PBS. (Only for Reference)
Cas No.
126544-47-6
分子式
C32H44O7
分子量
540.69
别名
Alvesco;Omnaris;RPR251526;Zetonna;环索奈德
储存和溶解度
Ethanol:54.1 mg/mL (100 mM)
DMSO:54.1 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years