OAC1 是一种能够激活 OCTamer 结合转录因子 4 的化合物。 它提高了 iPSC 重编程效率并加速重编程过程。

OAC1 is a compound activating Octamer-binding transcription factor 4 (Oct4). It enhances the iPSC reprogramming efficiency and accelerates the reprogramming process.

OAC1似乎通过增加Oct4-Nanog-Sox2三联体和Tet1（已知参与DNA去甲基化的基因）的转录来提高重编程效率。 OAC1不抑制p53-p21通路或激活Wnt-β-catenin信号。OAC1可用于增强体细胞重编程为多能状态。1 μM OAC1通过激活Oct4和Nanog启动子驱动的荧光素酶报告基因增强重编程效率。此外,OAC1增强四重编程因子（Oct4,Sox2,c-Myc和Klf4）处理的小鼠胚胎成纤维细胞（MEF）中多能干细胞（iPSC）重编程效率并加速重编程过程。

Enzymology: Kinesin motor domains are expressed in Escherichia coli BL21(DE3) and purified. CENP-E proteins includes residues 2–340 with a carboxyl-terminal 6-his tag. All studies using MT are conducted in PEM25 buffer [25 mM PipesK+ (pH 6.8), 2 mM MgCl2, 1 mM EGTA] supplemented with 10 μM paclitaxel. The IC50 for steady-state inhibition is determined at 500 μM ATP, 5 μM MT, and 1 nM CENP-E in PEM25 buffer.

The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.(Only for Reference)

300586-90-7

C14H11N3O

237.262

BAS 00287861

DMSO:23.7 mg/mL (100 mM)
Ethanol:23.7 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years