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GW 4064
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
GW 4064图片
CAS NO:278779-30-9
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
50 mg电议
100 mg电议
200 mg电议
1 mL*10 mM(in DMSO)电议

产品介绍
GW 4064 是一种有效的法尼醇 X 受体 (FXR) 激动剂,EC50为 65 nM。

产品描述

GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM).

体外活性

在Fisher大鼠中,口服给药GW 4064(ED50 = 20 mg/kg),能够降低大鼠血清中的甘油三酯.

体内活性

在小鼠FXR表达载体转染的CV-1细胞中(EC50=80 nM),在人FXR表达载体转染的CV-1细胞中(EC50=90 nM),GW 4064作为选择性非甾体FXR激动剂,能够发挥激动作用。

细胞实验

GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2].

Cas No.

278779-30-9

分子式

C28H22Cl3NO4

分子量

542.84

储存和溶解度

Ethanol:5.4 mg/mL (10 mM)
DMSO:54.3 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years