Necrostatin-34 (10?μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation. Necrostatin-34 may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase. Necrostatin-34 shows IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively.

375835-43-1

C18H16N4O2S2

384.48

2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4

DMSO:125 mg/mL (325.11 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years