NSC59984 是 p53 通路激活剂，可诱导突变 p53 蛋白降解和 p73 活化。它通过 Mdm2 和泛素-蛋白酶体途径而诱导突变型 p53 蛋白降解。

NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.

NSC59984 specifically restores p53 pathway signaling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. NSC59984 also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells. [1]

In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1]

Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.(Only for Reference)

803647-40-7

C12H15N3O4

265.269

DMSO:49 mg/mL (184.7 mM)
Ethanol:49 mg/mL (184.7 mM)
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years