Cell experiment:

Studies are performed in rabbit renal brush border membrane vesicles in which the uptake of radiolabeled GlySar is examined in the absence and presence of captopril, enalapril, enalaprilat, fosinopril, lisinopril, quinapril, quinaprilat, ramipril and Zofenopril[1].

Animal experiment:

Male and female mice weighing 8 to12 g (22 to 26 days old) or 20 to 28 g (48 to 56 days old) are used. Mice are exposed to auditory stimulation, 45, 60 or 120 min following intraperitoneal (i.p.) administration of ACE inhibitors (including Zofenopril) (10 to 100 mg/kg) or vehicle and 45 min following i.p. injection of the AEDs studied. All ACE inhibitors are suspended in a 1% solution of Tween 80 before administration[2].

Zofenopril Calcium (SQ26991) is an antioxidant that acts as an angiotensin-converting enzyme inhibitor.Target: ACEZofenopril Calcium is a pro-drug designed to undergo metabolic hydrolysis yielding the active free sulfhydryl compound zofenoprilat, which is an angiotensin converting enzyme (ACE) inhibitor [1]. Zofenopril Calcium promotes the regeneration of peripheral nerve injuries in rat models [2]. Zofenopril Calcium increases SR calcium cycling and stimulates active calcium uptake into the SR [3].

References:
[1]. Dal Bo, L., P. Mazzucchelli, and A. Marzo, Assay of zofenopril and its active metabolite zofenoprilat by liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B Biomed Sci Appl, 2000. 749(2): p. 287-94.
[2]. Kalender, A.M., et al., Effect of Zofenopril on regeneration of sciatic nerve crush injury in a rat model. J Brachial Plex Peripher Nerve Inj, 2009. 4: p. 6.
[3]. Frascarelli, S., et al., Effects of zofenopril on cardiac sarcoplasmic reticulum calcium handling. J Cardiovasc Pharmacol, 2009. 54(5): p. 456-63.