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Guanidine hydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Guanidine hydrochloride图片
CAS NO:50-01-1
包装与价格:
包装价格(元)
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
Aminoformamidine Hydrochloride
Guanidinium chloride
Guanidine HCl
Aminoformamidine HCl
盐酸胍
产品介绍
Guanidine hydrochloride 是一种强离液剂和一种蛋白质的强变性剂。

产品描述

Guanidine hydrochloride is a strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant.

体外活性

Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. [1] Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (>1 M) causes co-operative unfolding of the molten globule state. [2] Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GWaterapp. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. [3] Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast. [4] 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. [5]

Cas No.

50-01-1

分子式

CH6ClN3

分子量

95.53

别名

Aminoformamidine Hydrochloride;Guanidinium chloride;Guanidine HCl;Aminoformamidine HCl;盐酸胍

储存和溶解度

DMSO:198.9 mM
H2O:198.9 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years