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GJ103 sodium salt
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
GJ103 sodium salt图片
CAS NO:1459687-96-7
包装与价格:
包装价格(元)
2 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
1 mL*10 mM(in DMSO)电议

产品介绍
GJ103 sodium salt 是 GJ072 的活性类似物,它是一种通读化合物 (RTC)。

产品描述

GJ103 sodium salt is an active analog of GJ072, a read-through compound.

体外活性

GJ072 induces ATM kinase on both TGA and TAG stop codons and restored ATMpSer1981 autophosphorylation and SMC1pSer966 transphosphorylation as measured by FACS. GJ072 is active in A-T cells with a homozygous TAA mutation. GJ072 is able to induce detectable full-length ATM protein in treated A-T cells. Early structure-activity relationship studies generates eight active analogs of GJ072. Some GJ072 analogs (e.g., GJ103, GJ106, GJ109, and GJ111) consistently demonstrates their activities in all three PTCs by both FCATMpSer1981 and IRIF assays. GJ071 and GJ072 and some of their analogs (such as GJ103) have similar read-through activity as RTC13 or RTC14, but are more tolerable than RTC13 and RTC14 to A-T cells. GJ103 does not show obvious cytotoxicity in A-T cells at concentration as high as 300 μM.

体内活性

GJ103 sodium salt is water soluble, which will enable us to evaluate its in vivo activity by systematic administration.

细胞实验

Cell proliferation assay is used to measure cytotoxicity. Cells are seeded into a flat-bottom 96-well plate, including control wells containing complete growth medium alone as blank absorbance readings. After RTC treatment (GJ103), activated-XTT Solution is added into each well, and the cells are returned to the cell culture incubator for 12-14 hours. The absorbance is measured at 480 nM with relevant 630 nM to assess nonspecific readings.

Cas No.

1459687-96-7

分子式

C16H13N4NaO3S

分子量

364.35

储存和溶解度

H2O:Soluble
DMSO:34 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years