Orotic acid 是一种嘧啶核苷酸和 RNA 生物合成的前体,从线粒体二氢乳清酸脱氢酶 (DHODH) 中释放,通过细胞质 UMP 合酶转化为 UMP。它可引起大鼠肝脂肪变性和肝肿大,是尿素循环异常的常规新生儿筛查中的一种测量指标。
产品描述
Orotic acid is an intermediate product in pyrimidine metabolism.
体外活性
Orotic acid increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1) [1].
体内活性
Male Fischer 344 rats (130-150 g) to two-thirds PH in the absence or in the presence of Orotic acid (a 300-mg tablet of Orotic acid methyl ester implanted intraperitoneally at the time of two-thirds PH). treatment with Orotic acid resulted in a near-100% inhibition of RNR induced by two-thirds PH in rat liver, as monitored by enzyme activity and protein level [2]. Feeding 1% Orotic acid with diet decreased the phosphorylation of AMPK and increased the maturation of SREBP-1 and the expression of SREBP-responsive genes in the rat liver. Orotic acid-induced lipid accumulation was also completely inhibited by rapamycin. Mouse hepatocytes and mice were resistant to Orotic acid-induced lipogenesis because of little if any response in AMPK and downstream effectors [3].
Cas No.
65-86-1
分子式
C5H4N2O4
分子量
156.097
别名
乳清酸;Vitamin B13;6-Carboxyuracil
储存和溶解度
DMSO:12.8 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years