ITSA1 是一种具有膜渗透性的HDAC激活剂，抵消曲古抑菌素 A (TSA) 诱导的细胞周期停滞，组蛋白乙酰化和转录水平。

ITSA-1 is membrane permeable and specifically suppresses TSA inhibition of HDAC (histone deacetylase), but shows no activity towards other HDAC inhibitors.

A549 cells ( 75% confluent) in 10-cm dishes are treated with DMSO (0.1%), TSA (500 nM), or tubacin (2 μM) for 4.5 h, and then either DMSO (0.1%) or ITSA1 (50 μM) is added for an additional 17.5 h. Samples are rinsed in PBS, trypsinized, and fixed for 2 h in 70% ethanol 30% PBS (4°C). Cells then are washed once in PBS and stored overnight in PBS (4°C).(Only for Reference)

200626-61-5

C13H7Cl2N3O

292.12

ITSA1

DMSO:54 mg/mL (184.9 mM)
H2O:<1 mgml
Ethanol:5 mg/mL (17.12 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years