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Nitrocefin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Nitrocefin图片
CAS NO:41906-86-9
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
25mg电议
500mg电议

产品介绍
Nitrocefin 是一种显色 β-内酰胺酶底物,当 β-内酰胺环中的酰胺键被 β-内酰胺酶水解时,其颜色会从黄色变为红色。
Cas No.41906-86-9
别名头孢硝噻吩
化学名(6R,7R)-3-((E)-2,4-dinitrostyryl)-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Canonical SMILES[H][C@]1([C@@H]2NC(CC3=CC=CS3)=O)SCC(/C=C/C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)=C(C(O)=O)N1C2=O
分子式C21H16N4O8S2
分子量516.50
溶解度20mg/ml in DMSO, 20mg/mL in DMF
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Nitrocefin is a chromogenic cephalosporin substrate which is used for detection of β-lactamases. β-lactamases are enzymes produced by various microbes that mediate resistance to β-lactam antibiotics such as penicillins and cephamycins. Comparing to other methods for β-lactamases detection, nitrocefin is more sensitive while using few materials and inexpensive equipment. The color change by nitrocefin can be followed quantitatively by measuring changes in absorption between 380 to 500 nm region[1].

Reference:
[1] O'Callaghan, Cynthia H et al. Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate. Antimicrobial Agents and Chemotherapy. 1972 Apr; 1(4):283-288.