Azithromycin dihydrate是一种大环内酯抗生素，可研究细菌感染。

Azithromycin dihydrate is a semi-synthetic macrolide antibiotic structurally related to ERYTHROMYCIN. It has been used in the treatment of Mycobacterium avium intracellulare infections, toxoplasmosis, and cryptosporidiosis.

Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation, and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 which is sufficient to reduce viral load, but not RIG-I in exacerbating mice[2].

THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

117772-70-0

C38H72N2O12

785.026

CP-62993 dihydrate;阿奇霉素二水合物;阿奇霉素二水物;Azithromycin dihydrate

H2O:10 mg/mL (12.73 mM)
DMSO:93 mg/mL (118.5 mM)
Ethanol:93 mg/mL (118.5 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years