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MY-875
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
MY-875图片
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
MY-875 是一种竞争性的微管蛋白聚合 (microtubulin polymerization) 抑制剂,IC50值为 0.92 μM。MY-875 通过靶向秋水仙碱结合位点抑制微管蛋白聚合,同时可以激活 Hippo 通路。MY-875 可以诱导细胞凋亡 (apoptosis),具有抗癌活性。
生物活性

MY-875 is a competitivemicrotubulin polymerizationinhibitor with anIC50value of 0.92 μM. MY-875 inhibits microtubulin polymerization by targeting colchicine binding sites and activates theHippopathway. MY-875 inducesapoptosisand has anticancer activity[1].

体外研究
(In Vitro)

MY-875 (0-80 μM, 48 h) has significant anti-proliferative activity against cancer cells[1].
MY-875 (1-10 μM) can inhibit microtubule protein polymerization with an IC50value of 0.92 μM while inhibiting alkylation of β-tubulin and the formation of EBI-β-tubulin adduct bands in a dose-dependent manner[1].
MY-875 (0-45 nM, 48 h) can induce the phosphorylation state of MST (Ste20-like kinases) and LATS (large tumor suppressor kinases), leading to YAP (Yes-associated protein) degradation in a dose-dependent manner[1].
MY-875 (0-45 nM, 24 h) significantly inhibits cell colony-forming ability, arrests cells in the G2/M phase and induces cell apoptosis in a dose-dependent manner[1].

Cell Proliferation Assay[1]

Cell Line:MGC-803, HCT-116, KYSE450, HGC-27, SGC-7901cell lines
Concentration:0-80 μM
Incubation Time:48 hours
Result:Inhibited the proliferation of MGC-803, HCT-116, KYSE450, HGC-27 and SGC-7901 cells with the IC50values of 0.027, 0.055, 0.067, 0.033 and 0.025 μM, respectively.
Showed strong inhibitory effect on other tumor cell lines with the IC50values less than 0.1 μM, such as DU145, A549, MCF-7, etc.

Cell Cycle Analysis[1]

Cell Line:MGC-803, SGC-7901 cell lines
Concentration:0-45 nM
Incubation Time:24 hours
Result:Increased the percentage of cells in G2/M phase from 19.38% to 76.97% in MGC-803 cells and from 7.04% to 80.89% in SGC-7901 cells, respectively at 45 nM.

Apoptosis Analysis[1]

Cell Line:MGC-803, SGC-7901 cell lines
Concentration:0-45 nM
Incubation Time:48 hours
Result:Induced apoptotic cells from 21.96% to 76.08% in MGC-803 cells and from 9.28% to 63.51% in SGC-7901 cells, respectively at 45 nM.
Reduced expression of anti-apoptotic proteins c-IAP1, Bcl-xL and Mcl-1.
分子量

387.43

性状

Solid

Formula

C21H25NO6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : ≥ 250 mg/mL(645.28 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.5811 mL12.9056 mL25.8111 mL
5 mM0.5162 mL2.5811 mL5.1622 mL
10 mM0.2581 mL1.2906 mL2.5811 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。