iNOs-IN-3 (Compound 2d) 是一种具有口服活性的一氧化氮合成酶 (iNOS) 抑制剂 (IC50=3.342 μM)。iNOs-IN-3 具有抗炎活性，可用于 LPS 诱导的急性肺损伤 (ALI) 研究。
| 生物活性 | iNOs-IN-3 (Compound 2d) is an orally active nitric oxide synthase (iNOS) inhibitor (IC50=3.342 μM). iNOs-IN-3 shows anti-inflammatory activity and can be used in LPS-induced acute lung injury (ALI) research[1]. |
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体外研究
(In Vitro) | iNOs-IN-3 (25 μM; 24 h) inhibits LPS-induced RAW 264.7 cells[1].
iNOs-IN-3 (12.5 μM; 24 h) can decrease the expression of iNOS[1].
Cell Viability Assay[1] | Cell Line: | RAW 264.7 microphages | | Concentration: | 25 μM | | Incubation Time: | 24 hours | | Result: | Showed higher inhibitory activity (IC50=14.72 μM) in LPS-induced RAW 264.7 cells. |
Cell Viability Assay[1] | Cell Line: | RAW 264.7 microphages | | Concentration: | 12.5 μM | | Incubation Time: | 24 hours | | Result: | Inhibited the LPS-induced mRNA expression of iNOS obviously. |
Cell Viability Assay[1] | Cell Line: | RAW 264.7 microphages | | Concentration: | 12.5 μM | | Incubation Time: | 24 hours | | Result: | Inhibited the expression of TNF-α, IL-6, and IL-1β at 12.5 μM. |
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体内研究
(In Vivo) | iNOs-IN-3 (oral administration; 12.5 mg/kg; once) treatment shows anti-inflammatory activity against xylene-induced ear edema in mice[1].
iNOs-IN-3 (oral administration; 3.125 mg/kg, 6.25 mg/kg, 12.5 mg/kg; once) protects against LPS-induced acute lung injury[1].
| Animal Model: | Xylene-induced mice[1] | | Dosage: | 12.5 mg/kg | | Administration: | Oral administration; 12.5 mg/kg; once | | Result: | Showed better activity than the positive control. |
| Animal Model: | LPS-induced acute lung injury (ALI) mice[1] | | Dosage: | 3.125 mg/kg, 6.25 mg/kg, 12.5 mg/kg | | Administration: | Oral administration; 3.125 mg/kg, 6.25 mg/kg, 12.5 mg/kg; once | | Result: | Attenuated the pathological lesions dose-dependently, such as decreased inflammatory infiltration and pulmonary congestion. Inhibited LPS-induced lung edema dose-dependently. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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